TABLE 3.
lac expression from control promoters osmY and csiDa
| lac fusion | β-Galactosidase sp act for rpoS+ and rpoS strains in medium at tempb:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| K-tryptone
|
LBON
|
|||||||||
| 30°C
|
10°C
|
30°C
|
10°C
|
|||||||
| w.t. | rho | hns | w.t. | rho | w.t. | rho | hns | w.t. | rho | |
| osmY::lac | 376 (14) | 171 (30) | 287 (NDc) | 587 (20) | 334 (216) | 43 (12) | 78 (ND) | 257 (12) | 835 (36) | 489 (ND) |
| csiD::lac | 522 (64) | 195 (12) | 372 (ND) | 251 (33) | 258 (18) | 110 (10) | 80 (4) | 96 (4) | 37 (10) | 152 (39) |
a
The following GJ strains were used in the experiment, given in the order wild type (w.t.), rho mutant, and hns mutant for each with the isogenic rpoS derivatives indicated in parentheses: osmY::lac, 888 (2734), 889 (891), and 2741 (2751); csiD::lac, 884 (2733), 885 (887), and 2739 (2750).
b
Cultures of the various rpoS+ and isogenic rpoS strains were grown in trimethoprim-supplemented K-tryptone or LBON medium at 30 or 10°C for β-galactosidase assays. Enzyme specific activity values are reported in Miller units (35). Values in parentheses are for rpoS derivatives.
c
ND, not determined.