TABLE 4.
Strain | Mutation | β-Galactosidase sp act at temp:
|
|
---|---|---|---|
30°C | 10°C | ||
GJ862 | Nil | 123 | 839 |
GJ2746 | hfq-1 | 24 | 243 |
GJ2745 | hfq-2 | 85 | 750 |
Cultures of the indicated strains carrying plasmid pHYD275 were grown in trimethoprim-supplemented K-tryptone medium at 30 or 10°C for β-galactosidase assays. Enzyme specific activity values are reported in Miller units (35).