Table 1.

Discussed approaches and their characteristics.

Strategy	Method	Invasiveness	Labeling time	Enrichment	Multiplexing	Degradation analysis	Spatial resolution	Reference
Puromycin-based	PUNCH-P	–	15 min	+	No	–	−−	[10]
	OPP	–	2 h	+	No	–	−−	[11]
	OPP-pSILAC	–	2 h	+	No	–	−−	[12]
	QOT	+	15 min	+	Yes	–	−−	[13]
BONCAT-based	AHA/HPG	++	Hrs/days	+	No	+	−−	[17]
	ANL	–	Days (in vivo)	+	No	+	+	[43, 44, 45, 46∗]
	QuaNCAT	++	2–4 h	+	No	+	−−	[23]
	MITNCAT	++	15 min	+	Yes	+	−−	[40]
	HILAQ	++	1 h	++	No	+	−−	[24]
	PhosID	++	4–24 h	++	No	+	−−	[37]
SILAC-based	Dynamic SILAC	+++	6 h/days	N/A	No	++	−−	[47,48]
	mPDP	+++	3–48 h	N/A	Yes	++	−−	[50]
	MePROD	+++	2 h	N/A	Yes	++	−−	[49]

QOT, quantitative OPP tagging; MITNCAT, multiplex isobaric tagging/noncanonical amino acid tagging; HILAQ, heavy isotope-labeled AHA quantification; mPDP, multiplexed proteome dynamics profiling; MePROD, multiplexed enhanced protein dynamics.

Beneficial attributes are represented with ‘+’, whereas limitations are indicated as ‘−’. ‘Invasiveness’ describes the adverse effect of the technique on the system under investigation.