Table 3.
Summary of the mechanical devices/systems used in each study, their uncultured cell concentrations, viability (where applicable) and analytical techniques used
| Device/ System used (Author) | Adipose donor site | Harvest technique and manipulation of lipoaspirate prior to insertion in device/ system | Volume processed (ml) | Cell Concentration (x106/ml of lipoaspirate) | Cell Viability (%) | Estimated total cell yield of product (x106)a | Laboratory analysis used to quantify cell numbers (after device/system processing) | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Final volume of product (ml) | Enzyme use | Centrifugation | Filtration | Washing | Other Mechanical | Culture medium/ FBS/ Antibiotic | Counting Device | ||||||
| Adinizer (Copcu et al. [18]) | Abdomen | Harvested with 2.8mm diameter cannula with tumescent solution and adrenaline. Predilution with saline in 50% of samples tested | 5-20 | 1.22b | 92.75b | 1.13- 13.6 (Depending on volume used) | Y | LunaStem device | |||||
| 1-12 (Variable) | |||||||||||||
| Adiprep (Dragoo et al. [27]) | Knee fat pad | Harvested during arthroscopy into AquaVage system. Then subjected to fractionisation and syringe emulsification. | 30 | 0.486c | 69.03c | 0.99 (Mean) | Y | Y | Y | Y | Haemocytometer | ||
| ~2.95 (Mean) | |||||||||||||
| Fastem (Domenis et al. [24]) | Abdomen, hips and trochanter region | Harvesting procedure not mentioned. ‘Standardised procedural protocol’ not described. | No data | 0.444 to 1d | - | N/A | Y | Y | Y | No data | |||
| Fastem and MyStem (Gentile et al. [29]) | No data | Harvesting procedure not mentioned. | 80 | 0.03 and 0.005 | 98e | 0.29 and 0.049 | Y | Y | Y | Haemocytometer | |||
| 10 | |||||||||||||
| Hy-Tissue SVF (Busato et al. [12]) | Abdomen | Harvested with 11G cannula with Klein tumescence solution, followed by decantation | 25-30 | 0.041 | - | N/A | Y | Y | CytoSMART counter | ||||
| No data | |||||||||||||
| Lipocube Nano & Tulip Nanotransfer (Cohen et al. [17]) | No data | Harvested with 2.4mm diameter cannula and then cleaned with Ringer’s lactate, sedimented and decanted. | 10 | 2.24 and 1.44 | 96.05 | N/A | Y | Y | Y | Muse Flow Cytometer | |||
| No data (‘Pellet’ used) | |||||||||||||
| Lipocube SVF (Tiryaki et al. [66]) | Hip | Harvested with 3.5mm diameter cannula then decanted. | 20 | 0.94 | 97.55 | N/A | Y | Y | MuseCell Analyzer | ||||
| No data (‘Pellet’ used) | |||||||||||||
| Lipogems (Vezzani et al. [75]) | Abdomen | Harvested with 17G cannula either manually or vacuum assisted and mixed with saline | 60 | 0.027 | - | N/A | Y | Y | Y | Y | Y | Haemocytometer | |
| 20-30 | |||||||||||||
| Lull pgm (Morselli et al. [42]) | Abdomen | Harvesting procedure not mentioned. ‘Negative pressure’- not clarified. | 30 | 2.4 | - | N/A | Y | Y | Y | Y | Y | Cell Coulter counter | |
| 10 | |||||||||||||
| MyStem (Cicione et al. [16]) | No data | Harvested with MyStem 1.8mm blunt- tip cannula. Process not reported. | 17-50 | 0.6 | 75.87 | 3.6- 10.7 (Depending on introduced volume) | Y | NucleoCounter | |||||
| 8-23.5 (Variable) | |||||||||||||
| MyStem (Tarallo et al. [65]) | Abdomen | Harvested with local anaesthetic. ‘Standard protocol’- not described | 30 | 0.83 | 74.3 | 0.62- 4.3 | Y | Y | Y | NucleoCounter | |||
| 1-7 (Variable) | |||||||||||||
| Puregraft (Streit et al. [63]) | Abdomen | Harvested with 3.5mm diameter cannula with tumescent solution. | 50 | 0.198 | 60 | N/A | Y | Y | Y | Y | Haemocytometer | ||
| No data (Pellet used) | |||||||||||||
| Rigenera (Dai Pre et al. [20]) | Thigh and Abdomen | Harvesting procedure not mentioned. Lipoaspirate mixed with equal volume of culture medium, FBS and antibiotics. | 4 | 21 | - | N/A | Y | Y | Y | Tryptan blue exclusion assay | |||
| 4 | |||||||||||||
| Transpose RT (Winnier et al. [77]) | No data | Harvested with ‘standard procedure’- not described. Lipoaspirate mixed with lactated Ringer solution | 25 | 0.084 | 61.7 | 0.16 | NucleoCounter | ||||||
| 3 | |||||||||||||
| Tulip Nanotransfer (Sese et al. [61]) | Abdomen | Harvested with Carraway Harvester cannula with tumescent fluid, then washed with saline. | 20 | 6.63 | 76.8 | 50.9 | Y | Y | NucleoCounter | ||||
| 10 | |||||||||||||
a Estimated total cell yield= Volume of product (ml) X Cell concentration (x106/ml of lipoaspirate) X % Cell viability
b Value given is an average obtained from the four different protocols used in the study
c Figures from Layer 2 which resulted in the highest numbers
d Enrichment performed in only 50% of lipoaspirate sample
e Generalised figure for the study overall, not specific to either device/system