FIG. 3.

Wild-type genetic organization of the 23.7-kbp region carrying the genes involved in ETBE degradation (A) and restriction map of the 9.3-kbp BamHI fragment of the ETBE-negative mutant (B). The eth genes encode a putative transcriptional activator (ethR), a ferredoxin reductase (ethA), the ETBE-inducible cytochrome P-450 (ethB), a ferredoxin (ethC), and an ETBE-inducible unknown protein (ethD). Based on protein alignments, the other ORFs shown correspond to a resolvase (tnpR), transposase (tnpA), a two-component system response regulator (truncated orf1′), integral membrane protein (orf2), sodium:solute symporter (orf3), membrane protein (truncated orf7′), and three unidentified proteins (orf4, orf5, and orf6).