Skip to main content
. 2022 Oct 10;13:5947. doi: 10.1038/s41467-022-33745-4

Fig. 2. AAV.cc47 transduces mouse heart, skeletal muscle, and brain more efficiently than AAV9 following systemic administration.

Fig. 2

a 8–10 week old C57/B6 mice (n = 3) were injected intravenously at a dose of 5e13vg/kg with AAV9 (red) or AAV.cc47 (blue) and organs were harvested 4 weeks post injection. Vectors delivered a self-complementary AAV genome with the chicken-beta actin hybrid (CBh) promoter driving mCherry reporter expression. b Representative images of native mCherry fluorescence in heart, tibialis anterior and liver. c Representative images of native mCherry fluorescence in whole brain, cerebellum (CB), hippocampus (HC), and cortex (CTX). d Quantification of native mCherry fluorescence intensity in heart (P < 0.0386), tibialis anterior (P < 0.0001), liver (P < 0.0172), and brain (P < 0.0172). Brain mCherry fluorescence intensity was measured from rostral, midbrain, and caudal brain regions, and are summarized as a single brain tissue. e Quantification of percent neurons transduced in the CB (P < 0.0001), HC (P < 0.0015), and CTX (P < 0.0045) of C57/B6 mice was determined via immunofluorescence staining of mCherry and NeuN proteins and taking the ratio of total mCherry+ neuron counts to total NeuN+ cell counts in each brain region. Fifty micrometers thick cross sections were obtained via a vibratome for the heart, liver, and brain, while 7 μm thick cross sections were obtained for tibialis anterior via cryostat. For all quantification, 2 sections per mouse and 3 images per section (a total of 6 images) were used to quantify fluorescence intensity and cell counts using ImageJ. Each dot represents an individual mouse for fluorescence intensity, and for cell counting each dot represents an individual mouse, fold change is listed above significance, the dash and bars represent the mean value and error bars represent the standard error mean. Statistical significance was determined by a two-tailed Student t Test. *P < 0.05; ** P < 0.01; ***P < 0.001; ****P < 0.0001; ns not significant. Source data are provided as a Source Data file.