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. 2022 Sep 26;13:953502. doi: 10.3389/fimmu.2022.953502

Figure 2.

Figure 2

Evaluation of IFN-γ production after stimulation or not of circulating peripheral blood cells in healthy donors. (A) Plasma cytokine levels in seven healthy donors after in vitro nonspecific stimulation of immune cells by anti-CD3, or TLR7/8 agonist, or both anti-CD3 and TLR7/8 agonist, or no stimulation. IFN-γ production was mainly increased after TLR7/8 agonist stimulation and partially after anti-CD3 stimulation. (B) Representative plots of IFN-γ production by CD4+ T cells, CD8+ T cells, NK cells and B cells after stimulation or not with TL7/8 agonist and/or anti-CD3. As expected, after nonspecific stimulation CD4+ T, CD8+ T and NK cells produce more IFN-γ, but not B cells. The level of IFN-γ measured on the same individual and in the same conditions with no stimulation and after nonspecific stimulation with anti-CD3, TLR7/8 agonist, or both was 4.9, 300.2, 466.3 and >1000.0 IU/mL, respectively. *p<0.05; **p<0.01; ns, not significant. IFN, interferon; TLR, Toll-like receptor.