TABLE 4.
Effects of spo0E on sporulation of spo0A mutants
| Strainsa (spo0E+/Δspo0E) | spo0A allele | Other genotype | % Sporulationb
|
|
|---|---|---|---|---|
| spo0E+ | Δspo0E | |||
| JQ62/JQ63 | spo0A+ | Pspac-kinC | 1.0 × 10−6 | 3.0 × 10−4 |
| JQ221/JQ263 | spo0A(N12K) | Pspac-kinC | 2.0 × 10−3 | 44 |
| JQ397/JQ370 | spo0A(E14A) | Pspac-kinC | 1.5 × 10−3c | 64 |
| JQ367/JQ363 | spo0A(E14A) | ΔkinC | 9.4 × 10−5c | 0.78 |
| JQ395/JQ396 | spo0A(P60S) | ΔkinC | 2.4 | 1.6 |
| JQ82/JQ95 | spo0A(Q90R) | Pspac-kinC | 7.6 × 10−2 | 46 |
| JQ387/JQ388 | spo0A(D92Y) | ΔkinC | 0.33 | 0.24 |
a
All strains are ΔkinA ΔkinB Δspo0F Δspo0B. Strains carrying spo0A(P60S) and spo0A(D92Y) were unaffected by kinC, and therefore the assay was done in a ΔkinC background. Pspac-kinC was induced with 1 mM IPTG added when the culture was inoculated.
b
Strains were grown in DS medium. Wild-type (JH642) cultures produced 50 to 80% spores.
c
Data are from the experiment shown in Table 3.