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Effects of kinA, kinB, and kinC on sporulation of the spo0A(E14A) Δspo0E mutant
| Straina | Other genotypeb | % Sporulationc |
|---|---|---|
| JQ363 | ΔkinABC | 0.33 |
| JQ235 | Pspac-kinA ΔkinBC | 35 |
| JQ372 | Pspac-kinB ΔkinAC | 0.09 |
| JQ370 | Pspac-kinC ΔkinAB | 47 |
All strains are spo0A(E14A) Δspo0F Δspo0B Δspo0E.
Expression of kinases from the Pspac promoter was induced with 1 mM IPTG added when the culture was inoculated.
Strains were grown in DS medium. Wild-type (JH642) cultures produced 50 to 80% spores.
