Table 2.
Bioinformatic prediction of outer membrane proteins in Liberibacter crescens BT-1 and the LC–MS/MS results of proteins which were also identified from bacterial outer membrane fraction.
| Protein locus | β barrel predictors | Sub-cellular localization predictors | Lipoprotein predictors | LC–MS/MS (spectrum counts in the 3 biological replicates) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| BOMP | MCMBB | TMBETA DISC-RBF |
CELLO | PSORTb | SOSUIGramN | LIPO | LIPOP | 1 | 2 | 3 | |
| AGA63999.1 | – | – | – | Y | – | – | Y | – | 6 | 3 | 6 |
| AGA64002.1 | – | Y | Y | Y | – | – | – | – | |||
| AGA64040.1 | – | Y | Y | Y | Y | Y | – | – | 95 | 80 | 96 |
| AGA64234.1 | – | Y | Y | Y | Y | – | – | – | 23 | 17 | 14 |
| AGA64245.1 | – | – | Y | – | – | Y | Y | Y | |||
| AGA64332.1 | – | Y | Y | Y | – | Y | – | – | 1 | 3 | 1 |
| AGA64343.1 | – | – | Y | Y | Y | – | – | – | 27 | 23 | 28 |
| AGA64354.1 | – | Y | Y | Y | – | – | – | Y | 2 | 2 | 3 |
| AGA64387.1 | Y | Y | – | – | – | – | – | – | |||
| AGA64389.1 | Y | Y | Y | Y | Y | – | – | – | |||
| AGA64428.1 | – | – | Y | Y | – | Y | – | – | |||
| AGA64431.1 | – | – | – | – | – | – | Y | Y | |||
| AGA64432.1 | – | – | – | – | – | – | – | Y | |||
| AGA64438.1 | – | – | – | – | – | – | Y | – | |||
| AGA64470.1 | Y | Y | Y | Y | Y | Y | – | – | 13 | 16 | 14 |
| AGA64492.1 | – | Y | Y | – | – | – | – | – | |||
| AGA64501.1 | – | Y | Y | – | – | – | – | – | 3 | 4 | 3 |
| AGA64506.1 | – | Y | Y | Y | – | – | – | – | |||
| AGA64508.1 | – | Y | Y | Y | – | Y | – | – | |||
| AGA64510.1 | – | Y | Y | – | – | – | – | – | |||
| AGA64549.1 | – | – | – | – | – | Y | Y | Y | 8 | 5 | 6 |
| AGA64564.1 | Y | Y | – | – | Y | – | – | – | 6 | 9 | 3 |
| AGA64573.1 | – | – | – | – | Y | Y | Y | Y | 3 | 1 | 3 |
| AGA64585.1 | – | Y | Y | Y | – | – | – | – | |||
| AGA64591.1 | – | – | Y | – | Y | – | Y | Y | 13 | 9 | 13 |
| AGA64614.1 | Y | Y | Y | Y | Y | Y | – | – | 30 | 25 | 27 |
| AGA64636.1 | – | Y | Y | – | Y | – | – | – | 29 | 34 | 25 |
| AGA64650.1 | – | Y | – | – | Y | Y | Y | Y | 47 | 35 | 47 |
| AGA64651.1 | – | – | Y | Y | Y | Y | – | – | 4 | 5 | 1 |
| AGA64766.1 | – | Y | Y | – | – | – | – | – | |||
| AGA64823.1 | – | Y | Y | Y | – | – | Y | Y | 16 | 11 | 14 |
| AGA64892.1 | Y | Y | Y | Y | Y | Y | – | – | |||
| AGA64980.1 | – | Y | Y | Y | Y | Y | – | Y | 61 | 68 | 68 |
| AGA65052.1 | Y | Y | Y | Y | Y | Y | – | – | 54 | 47 | 61 |
| AGA65105.1 | – | Y | Y | – | Y | Y | Y | Y | 6 | 4 | 6 |
| AGA65110.1 | – | Y | Y | Y | – | – | – | – | |||
| AGA65128.1 | – | – | – | – | – | – | Y | Y | 5 | 2 | 1 |
| AGA65134.1 | – | – | – | – | – | – | – | Y | 8 | 8 | 12 |
| AGA65143.1 | Y | Y | Y | Y | Y | Y | – | – | 31 | 30 | 33 |
| AGA65153.1 | Y | Y | Y | Y | – | Y | – | – | 5 | 3 | 0 |
| AGA65154.1 | – | Y | Y | Y | Y | – | – | – | 10 | 9 | 9 |
| AGA65155.1 | – | – | – | – | – | – | Y | Y | |||
| AGA65174.1 | – | – | – | – | – | – | Y | Y | |||
| AGA65230.1 | – | – | – | – | – | – | Y | Y | |||
| AGA65239.1 | – | – | Y | Y | – | Y | – | – | |||
| AGA65242.1 | – | – | – | Y | – | – | – | Y | 2 | 2 | 0 |
| AGA65276.1 | – | Y | Y | Y | Y | – | – | – | 50 | 40 | 48 |
| AGA65326.1 | – | Y | – | Y | – | Y | – | – | 2 | 1 | 3 |
| AGA65331.1 | – | Y | Y | Y | – | – | – | – | 9 | 2 | 3 |
| AGA65241.1 | – | – | – | Y | – | Y | – | – | 25 | 21 | 18 |
Note: Y represent the protein is predicted to have β barrel domain, localized in outer membrane compartment or is a lipoprotein based on sequence analysis from different predictors.