TABLE 1.
Spot | N-terminal sequence | Locus | Protein | Protein description | Function |
---|---|---|---|---|---|
A8 | MLIVTKNPPVVGHDQ | PA3623 | NIpD | Outer membrane lipoprotein | Cell wall |
A6 | DDKVLHVVN-D–A | PA0300 | PotF2 | ABC transporter | Polyamine transport |
B9 | DVKIGVAGPMTDA | PA4913 | NIb | ABC transporter | Amino acid metabolism |
B2 | KEAETVQKLANVVIL | PA1337 | AnsB | Glutaminase asparaginase | Amino acid metabolism |
A4 | SAEKQK-GVHSEAGK | PA5171 | ArcA | Arginine deiminase | Amino acid metabolism |
A7 | AFNIHNRNLL | PA5172 | ArcB | Ornithine carbamoyltransferase | Amino acid metabolism |
B1 | MFSRDLTIAK-DA-L | PA4602 | GlyA3 | Serine-hydroxymethyltransferase | Amino acid metabolism |
B4 | -PATK-P | No match | |||
B3 | PATA-PAGKN | No match | |||
A5 | AD-IKI-GAQ-QD | No match |
The protein spots were N-terminally sequenced, and the proteins were identified by comparing the N-terminal amino acid sequence to that found on the P. aeruginosa website using the BLASTX program (1). The spot numbers correlate with the numbers indicated in Fig. 3. The locus designates the gene number in the genome of P. aeruginosa.
NI, not identified.