Figure 3.

Schematic representation of in vitro Phage Display Technology. (A) In phage display systems, antibody genes are linked to the amino terminus region of the phage minor coat protein pIII, as shown in the phagemid. When expressed, mature phages will incorporate the encoded fusion product, creating a link between antibody genotype and phenotype. (B) In vitro phage display, selection is composed of several steps: coating of the antigen or preparation of the cells; incubation of phage repertoire with antigen; washing to remove non-specific phages; and elution and reamplification of antigen-specific phages. The stringency of selection could be increased by the increase in the number of washing steps.