FIG. 5.

benA gene disruption in Rhodococcus sp. strain RHA1. (A) Strategy for gene disruption. Disruption of the benA gene was accomplished by a single crossover between the chromosomal benA gene and the pDA-tsr plasmid containing truncated benA whose amino and carboxyl termini were deleted. The sizes of BglII fragments containing the benA sequence are indicated. ≫ and ≪ represent amino-terminal (5′) and carboxyl-terminal (3′) deletions, respectively. (B) Southern blot analysis of benA insertion mutant strain RBD169. Lanes 1, 1-kb ladder marker; lanes 2, RHA1 total DNA digested with BglII; lanes 3, RBD169 total DNA digested with BglII. The benA gene fragment (left lanes) and the tsr gene fragment (right lanes) were used as probes.