Table 2.
Class | Name | Threshold (mg/100 g) | Content (mg/100 g) | TAV | |||||
---|---|---|---|---|---|---|---|---|---|
0 d | 7 d | 14 d | 21 d | 28 d | 35 d | MAX | |||
Organic acid | Succinic acid | 10.6 | 278.42 ± 2.06a | 273.22 ± 1.60b | 247.97 ± 3.79c | 238.70 ± 1.03d | 245.87 ± 0.85c | 280.87 ± 1.66a | 26.50 |
Free amino acids | Asp | 100 | ND | ND | ND | 6.16 ± 0.3.9a | 5.97 ± 0.29a | 6.40 ± 0.28a | 0.06 |
Thr | 260 | 8.95 ± 0.33a | 7.98 ± 0.10b | 6.80 ± 0.68c | 5.80 ± 0.11d | 5.13 ± 0.72d | 5.38 ± 0.12d | 0.03 | |
Ser | 150 | 8.06 ± 0.10a | 7.05 ± 0.06b | 6.13 ± 0.18c | 5.69 ± 0.15cd | 5.21 ± 0.68d | 5.48 ± 0.13d | 0.05 | |
Glu | 30 | 17.45 ± 0.16a | 15.60 ± 0.14b | 14.81 ± 0.68c | 13.41 ± 0.15d | 12.85 ± 0.77d | 13.14 ± 0.29d | 0.58 | |
Gly | 130 | 2.69 ± 0.02a | 2.49 ± 0.02b | 2.18 ± 0.14c | 2.10 ± 0.02cd | 2.01 ± 0.13cd | 2.10 ± 0.02d | 0.02 | |
Ala | 60 | 5.87 ± 0.01a | 5.05 ± 0.05b | 4.63 ± 0.33c | 4.31 ± 0.036c | 4.17 ± 0.15d | 4.60 ± 0.15d | 0.10 | |
Val | 40 | 7.6 ± 0.07a | 7.23 ± 0.05a | 6.51 ± 0.43b | 5.93 ± 0.14c | 5.55 ± 0.46c | 5.83 ± 0.14c | 0.19 | |
Met | 30 | 3.96 ± 0.01a | 3.58 ± 0.35a | 3.11 ± 0.19b | 2.81 ± 0.13bc | 2.48 ± 0.49bc | 2.76 ± 0.17c | 0.13 | |
Ile | 90 | 5.74 ± 0.05a | 5.55 ± 0.04a | 5.10 ± 0.36b | 4.71 ± 0.22bc | 4.33 ± 0.41c | 4.62 ± 0.12c | 0.06 | |
Leu | 50 | 22.46 ± 0.03a | 20.46 ± 0.15b | 18.90 ± 1.27c | 16.76 ± 0.52d | 15.33 ± 1.44d | 16.24 ± 0.47d | 0.45 | |
Tyr | – | 9.98 ± 0.07a | 8.87 ± 0.04b | 8.35 ± 0.51b | 7.67 ± 0.29c | 6.93 ± 0.63cd | 7.37 ± 0.28d | – | |
Phe | 90 | 20.17 ± 0.24a | 11.09 ± 0.22b | 12.41 ± 0.47b | 12.62 ± 0.10b | 12.65 ± 1.27b | 12.06 ± 1.98b | 0.22 | |
Lys | 50 | 13.94 ± 0.05a | 12.0 ± 0.12b | 11.13 ± 0.62bc | 10.44 ± 0.30bc | 9.34 ± 1.50bc | 10.80 ± 1.39c | 0.28 | |
His | 20 | 3.20 ± 0.02a | 2.82 ± 0.02b | 2.30 ± 2.03c | 2.10 ± 0.06c | 1.89 ± 0.14b | 1.77 ± 0.09b | 0.16 | |
Arg | 50 | 10.56 ± 0.10a | 9.55 ± 0.08ab | 8.69 ± 0.84bc | 8.61 ± 0.44bc | 7.39 ± 0.68bc | 8.61 ± 1.45c | 0.21 | |
Total | 140.72 ± 0.64a | 115.31 ± 6.97b | 111.06 ± 6.86bc | 107.06 ± 1.77bc | 100.60 ± 10.60bc | 106.92 ± 1.92c |
Values in the table are expressed as mean ± standard deviation (n = 3); Different superscripts of lowercase letters in the same line indicate significant difference between mean values (abc), p < 0.05.