FIG. 3.

Effects of an hha mutation on expression of hilA::Tn5lacZY after growth under repressing conditions. Cultures of wild-type (WT) (hilA::Tn5lacZY) or the hha mutant (hilA::Tn5lacZY hha::kan) were grown in LB medium with 1% NaCl under oxygen-limiting conditions (activating), LB medium with 1% NaCl with vigorous shaking (high oxygen), or LB medium with no NaCl under oxygen-limiting conditions (low osmolarity). β-Galactosidase of the hilA::Tn5lacZY reporter values were standardized, with the wild-type invasion after growth under inducing conditions being set to 100%.