Skip to main content
. Author manuscript; available in PMC: 2023 Sep 1.
Published in final edited form as: Mol Microbiol. 2022 Aug 15;118(3):258–277. doi: 10.1111/mmi.14966

Fig 9. Epifluorescence microscopic analysis of inner polysaccharide deposition in ysxE mutant sporangia during sporulation.

Fig 9.

Sporangia were harvested from hours t2 through t8 of sporulation, fixed with methanol, visualized either by phase-contrast microscopy (top row) or epifluorescence (bottom row). Cells were stained with Hoechst 33342 (to detect DNA, blue), polyclonal anti-BclA antibodies (to detect the exosporium, green) and WGA (to detect inner polysaccharide, magenta; once the exosporium encircles the entire spore (t6) WGA staining pattern is variable, indicated by the dashed line in the cartoon above the panels)