TABLE 2.

Primer used in this study.

Isolates	Primer	Primer sequence	Annealing temp.(°C)	Amplicon size (bp)
Universal 16S	27 F 1494 R	5′-AGAGTTTGATCCTGGCTCAG-3′ 5′-CTACGGCTACCTTGTTACGA-3′	53	1500
Pseudomonas aeruginosa MAPB-2	PA-ARHD F PA-ARHD R	5′-GGCCAGGCGAAGGACTATAT-3′ 5′-GTGCCGAGGGTATTCAGGTA-3′	57	157
Pseudomonas plecoglossicida MAPB-6	PP-ARHD F PP-ARHD R	5′-AGAAGCTTTTACCCTGCCCT-3′ 5′-GGAACGCATGAATCTGTCCC-3′	57	199
Brucella anthropi MAPB-9	BA-ARH F BA-ARH R	5′-GACCAGCTGGAGAAGCAGAT-3′ 5′-TGAACCCCTTCGACAGATTC-3′	57	161
Priestia megaterium MAPB-27	BM-ARHD F BM-ARHD R	5′-ACCGCACGTATTTTGGCATT-3′ 5′-CACCACTCACACGCTTCAAA-3′	57	202