FIG. 2.

Expression of GFP fusions from Pbad and induction of MinCD do not affect the level of FtsZ. Samples from the experiments in Fig. 1 and 3 were analyzed by immunoblotting using an anti-FtsZ polyclonal antibody. Lane 1, purified FtsZ and FtsZ-GFP; lanes 2 to 6, samples taken at the same time as the fluorescence pictures of Fig. 1 and 3; lane 2, sample from Fig. 3A (ZipA-GFP, no MinCD); lane 3, sample from Fig. 3B (ZipA-GFP plus MinCD); lane 4, sample from Fig. 1A (FtsZ-GFP, no MinCD); lane 5, sample from Fig. 1D (FtsZ-GFP plus MinCD); lane 6, sample from Fig. 1C (FtsZ-GFP, no MinCD).