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MinCD-induced division inhibition and the effect of multicopy ftsQAZ. Vector pJPB209 or the indicated derivatives were introduced into PB114 Δmin (λDB173), which expresses minCD under the control of Plac. One colony of each strain was resuspended in 300 μl of LB medium and serially diluted by 10. Samples (4 μl) were spotted on plates containing SPC and glucose with or without IPTG (as indicated) and incubated overnight at 37°C.
