TABLE 1.
Strains and plasmids used in this study.
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| PB114 (λDB173) | Δmin λPlac::minCD | 11 |
| JKD7.2 | ftsZ::kan recA56; FtsZ supplied by pKD3c | 10 |
| JKD7.2 (λDB173) | λDB173 lysogen of JKD7.2 | This study |
| PS269 | PB114 (λDB173/pSEB103) | This study |
| PS270 | PB114 (λDB173/pSEB104) | This study |
| PS294 | JKD7.2 (λDB173/pBEF0) | This study |
| PS293 | JKD7.2 (λDB173/pBEF2) | This study |
| PS309 | JKD7.2 (λDB173/pBEF9) | This study |
| PS310 | JKD7.2 (λDB173/pBEF100) | This study |
| PS311 | JKD7.2 (λDB173/pBEF114) | This study |
| PS299 | PB114 (λDB173/pJPB209) | This study |
| PS300 | PB114 (λDB173/pJPB222) | This study |
| PS301 | PB114 (λDB173/pJPB223) | This study |
| PS302 | PB114 (λDB173/pSEB25) | This study |
| PS298 | PB114 (λDB173/pSEB162) | This study |
| Plasmids | ||
| pBEF0 | Spcr; wild-type ftsZ on a pGB2 derivative | 4 |
| pBEF2 | Spcr; pBEF0 but contains ftsZ2 | 4 |
| pBEF9 | Spcr; pBEF0 but carries ftsZ9 | 4 |
| pBEF100 | Spcr; pBEF0 but carries ftsZ101 and ftsZ114 mutations | 4 |
| pBEF114 | Spcr; pBEF0 but carries ftsZ114 mutation (same as ftsZ103, sfiB103, or sfiB114) | 4 |
| pKD3c | Cmr; temperature-sensitive pSC101 derivative containing ftsAZ and envA | 2 |
| pSEB12 | Cmr; mini-F plasmid containing minCDE under the control of their own promoters | 26 |
| pSEB14 | Cmr; mini-F plasmid encoding minCD under the control of their own promoters | 26 |
| pJPB222 | Spcr; same as pJPB223 but ftsQAZ genes are in opposite orientation to the aadA gene; the level of expression of ftsQAZ is lower than in pJPB223 | Gift from Jean-Pierre Bouché |
| pJPB223 | Spcr; ftsQAZ cloned on pJPB209, aadA, and ftsQAZ are in the same orientation | 26 |
| PSEB25 | Spcr; pJPB223 with the PvuII fragment deleted, yielding an internal in-phase deletion of ftsA | Gift from Jean-Pierre Bouché |
| pSEB162 | Spcr; pJPB223 with the EcoRI fragment containing ftsZ deleted | This study |
| pSEB103 | Spcr; zipA-GFP under the control of PBAD on a pGB2 derivative | This study |
| pSEB104 | Spcr; ftsZ-GFP under the control of PBAD on a pGB2 derivative | This study |
| pJPB209 | Spcr; cloning vector derived from pGB2 | 26 |
| pGB2 | Spcr; cloning vector with the pSC101 origin of replication | 9 |