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Cholesterol supplementation rescues bunyavirus replication in polyamine-depleted cells. (A) Vero cells were treated with 1 mM DFMO for four days and subsequently supplemented with cholesterol for 8 h prior to infection with RVFV for 48 h. Viral titers were measured by the plaque assay. (B) BHK-21 cells were treated, infected, and analyzed as in A. NS not significant, *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s T-test comparing treatment to untreated conditions (N ≥ 3).
