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. 2001 Nov;183(22):6636–6644. doi: 10.1128/JB.183.22.6636-6644.2001

FIG. 1.

FIG. 1

GRA with the flhA promoter probe. (A) Lysate from PAK-Q harboring either pMMB67EH (vector control, lane 2) or pMMBQ (expressing FleQ, lane 3). The free probe was electrophoresed in lane 1. (B) Purified MBP-FleQ and MBP proteins. Lane 1 depicts the free probe, and lanes 2 to 4 and lanes 5 to 7 contain 1.1, 2.2, and 4.4 μg of MBP-FleQ and MBP, respectively. +, present; −, absent. (C) MBP-FleQ and cold flhA promoter or herring sperm DNA. Lanes 1 to 4 contain 5.6 μg of MBP-FleQ and cold flhA promoter fragment (lanes 2 and 3, 50 and 200 ng, respectively) or herring sperm DNA (lane 4, 1 μg). The arrow and arrowhead indicate the probe-FleQ complex and the free probe, respectively.