TABLE 2.
Assessment of the flhA promoter activity in the fleN, fleQ, and fleR mutant strains under induced expression of FleN from pMMBN
| Strain | Plasmid (promoter) | β-Galactosidase activity (mean ± SD) (Miller units) when cotransformed with:
|
|||
|---|---|---|---|---|---|
| pMMB67EH (vector control)
|
pMMBN (expressing FleN)
|
||||
| Uninduced | Induced | Uninduced | Induced | ||
| PAK-N | placΩA (flhA) | 88,943 ± 3,939 | 87,937 ± 2,125 | 3,626 ± 229 | 854 ± 63 |
| pMSZ5 (pilA) | 12,317 ± 863 | NDa | 11,665 ± 68 | ND | |
| pDN19lacΩ | 208 ± 2 | 234 ± 14 | 210 ± 19 | 270 ± 39 | |
| PAK-Q | placΩA (flhA) | ND | 570 ± 29 | ND | 585 ± 16 |
| PAK-R | placΩA (flhA) | ND | 7,890 ± 333 | ND | 856 ± 32 |
a
ND, not done.