TABLE 3.
Assessment of yeast cotransformants with different combinations of plasmids for a positive β-galactosidase filter assay and His+ phenotype
| Plasmid (protein expressed) | Resulta with plasmid (protein expressed):
|
||||
|---|---|---|---|---|---|
| pGBTN (FleN) | pGADN (FleN) | pGBT9 | pGAD424 | pGAD-M (FlgM) | |
| pGBTQ (FleQ) | ND | + (2.8 ± 0.28) | ND | − (not detectable) | ND |
| pGADQ (FleQ) | + (2.7 ± 0.14) | ND | − (not detectable) | ND | ND |
| pGBT9 | ND | − (not detectable) | ND | − (not detectable) | ND |
| pGAD424 | − (not detectable) | ND | − (not detectable) | ND | ND |
| pGBKT7-A (FliA) | ND | − (0.01 ± 0.0) | ND | ND | + (0.21 ± 0.06) |
| pGBKT7-M (FlgM) | ND | − (0.02 ± 0.0) | ND | ND | ND |
a
β-Galactosidase activity in Miller units (mean ± standard deviation), determined in a liquid culture assay, appear in parentheses. +, positive assay for both β-galactosidase and His+ phenotype; −, negative assay for both β-galactosidase and His+ phenotype; ND, assay not performed.