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Comparison of wild-type and mutated FleN proteins in their ability to downregulate the flhA promoter (placΩA) activity in a β-galactosidase reporter assay
| Plasmid | (protein expressed) | β-galactosidase activity (mean ± SD) (Miller units) |
|---|---|---|
| pMMBa | 8,422 ± 107 | |
| pMMBN | (FleN) | 1,123 ± 84 |
| pMMBN4 | (FleNΔ261–280) | 8,590 ± 56 |
| pMMBN5 | (FleNΔ271–280) | 8,256 ± 67 |
| pMMBN24K→Q | (FleN24K→Q) | 7,198 ± 92 |
Vector control, no protein expressed.
