FIGURE 2.

Effect of acute sleep deprivation on plasma small extracellular vesicles (EVs) in rats. Plasma samples were collected from rats carrying out one night of sleep deprivation and control group, and 100,000 g pellets were prepared. (A–C) Particle concentration and size were measured by nanoparticle tracking analysis. n = 10. (D) Protein content of the particles was analysed by Western blotting (n = 6) and (E) the signals were quantified. SC, Sleep control group; SD, Sleep deprivation group. Data are presented as mean ± SEM, *p < 0.05, ***p < 0.001.