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. 2001 Nov;183(22):6645–6653. doi: 10.1128/JB.183.22.6645-6653.2001

FIG. 6.

FIG. 6

Dependence on pH of the Na+/H+ antiporter activity of E. coli KNabc/pAM2 and KNabc/pAM3. Everted membrane vesicles were isolated from the E. coli strains KNabc/pAM2 (▴), KNabc/pAM3 (▪), and DH5α/pSK+ (○) as described in Materials and Methods. The assay was performed as outlined in the legend to Fig. 5 at the pH values indicated. (A) The initial rate of dequenching (ΔF/F) per minute and milligram of everted membrane vesicles is plotted against the pH. (B) The data obtained for the vesicles of the different strains are plotted each as percent respective maximal activity (100% = maximal quenching in each case) versus pH.

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