Fig. 1.

Exosomes from highly invasive breast cancers are more likely to promote cancer cell migration than less invasive ones. A Transmission electron microscopy of exosomes extracted from MDA-MB-231 and MCF7 breast cancer cell lines. Scale bar, 200 nm. B Particle size distribution of isolated exosomes. C Western blot analysis of Alix, TSG101 and CD63, marker proteins of exosomes isolated from MDA-MB-231 and MCF7 cells. D Exosomes were labelled using Dio stain and co-incubated with MCF7 and MDA-MB-231 cells for 3 h. Scale bar, 10 μm. E Wound healing experiments with MCF7 and MDA-MB-231 cells treated with MDA-MB-231 exosomes and MCF7 exosomes, respectively. Data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001