Fig. 3.

Downregulation of exosomal miR-7-5p expression significantly facilitates the migration and invasion of breast cancer cells. A Expression of miR-7-5p in the secreted exosomes of blank control, miR-7-5p mimic and suppressor transfected MDA-MB-231 cells was measured using qRT-PCR assay. B, C The expression of miR-7-5p in MDA-MB-231 cells and MCF7 cells after transfection of blank control, miR-7-5p mimics and inhibitors and their negative controls was measured using qRT-PCR assay. D, E Wound healing assay using blank control, miR-7-5p mimic and inhibitor and its negative control after treatment of MDA-MB-231 cells and MCF7 cells. F, G The migratory invasion ability of cells after treatment of MDA-MB-231 cells and MCF7 cells with blank control, miR-7-5p mimics and inhibitors and their negative control was examined using Transwell assay. Data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001