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. 2022 Oct 10;20:148. doi: 10.1186/s12958-022-01019-9

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — A Preantral follicle with 2–3 layers of granulosa cells collected after collagenase digestion of ovary (B) Individually isolated follicles seeded in HA gel on day 2 of culture. Follicles tended to aggregate during gelling process. C Day 4 of culture. Follicles growing in different planes within HA gel (D) HA-encapsulated on day 9 of culture. Antrum formation discernible (E, F) FL-cluster from fresh ovary embedded in HA gel shown at start of culture and on day 6. Granulosa cell proliferation evident and FL-cluster takes on an”organoid” appearance (G, H) FL-cluster mechanically isolated from vitrified ovary shown on day 2 and day 8 of culture