Table 1.

Comparison of in vitro growth of HA encapsulated follicles from fresh versus vitrified ovaries

	Fresh	Fresh	Frozen	P-Value
	FL-Isolated	FL-Cluster	FL-Cluster
	130	154	69
Antrum formation	51%(66/130)	53% (81/154)	-
Ovulated complexes after hCG	71% (92/130)	66% (101/154)	93% (64/69)	0.0001
GVBD (MI formation)	30% (28/92)	28% (28/101)	52% (33/64)	0.004
Maturation to MII	59% (54/92)	55% (56/101)	34%( 22/64)	0.007

This table contrasts outcomes between fresh and frozen ovaries. These data show that all outcome parameters for FL-Isolated and FL-Clusters from fresh ovaries were similar but differed significantly with results from frozen ovaries. The final maturation rate to MII was significantly lower with FL-Clusters from frozen ovaries versus fresh FL-Isolated or fresh FL-cluster