Table 2.
The effect of functional additives on cryopreservation of poultry semen
| Additive and proper concentration | Base extender and CPA | Beneficial effects | Fertility (Treatment/Control) | Reference |
|---|---|---|---|---|
| Flavonoid quercetin, 10 mmol/L | Beltsville extender, 3% glycerol | Increase sperm motility, membrane functionality, and mitochondrial activity | 64%/55% | [51] |
| Mito-TEMPO, 5 and 50 mmol/L | Lake extender, unspecified CPA | Decrease LPO; Increase mitochondria activity, acrosome integrity, and viability | 65%/48% | [52] |
| Serine, 4 mmol/L | BHSV extender, 6% DMF | Decrease LPO; Increase membrane integrity, acrosome integrity, and mitochondria activity | 90%/84% | [53] |
| SOD, 50 U/mL | Modified Beltsville extender, 3% glycerol | Increase sperm motility and velocity | NA | [54] |
| Coenzyme Q10, 1 and 2 mmol/L | Lake extender, 3% glycerol | Increase sperm viability, motility, membrane functionality, acrosome integrity, and mitochondria activity | 62%/42% | [55] |
| Melatonin, 1 and 10−3 mmol/L | EK extender, 6% DMA | Increase plasma membrane integrity, mitochondria activity, and motile sperm cell count | NA | [56] |
| Melatonin, 0.25 mg/mL | Undefined, 13.5% glycerol | Decrease oxidative stress level, increase acrosome integrity, plasma membrane integrity, and sperm motility | NA | [57] |
| L-Carnitine, 1 and 2 mmol/L | Beltsville extender, 3% glycerol | Decrease LPO; Increase sperm motility, viability, and membrane functionality | NA | [58] |
| Hyaluronic acid, 1 and 2 mmol/L | Beltsville extender, 3% glycerol | Decrease MDA; Increase sperm motility and acrosome integrity | 65%/40% | [59] |
| Vitamin E, 5 mg/mL | Modified Beltsville extender, 11% glycerol | Increase sperm motility, viability, and membrane integrity | NA | [60] |
| CAT, 100 IU/mL | Modified Beltsville extender, 11% glycerol | Increase sperm motility, viability, and membrane integrity | NA | [60] |
| CAT, 100 µg/mL | Modified Beltsville extender, 3% glycerol | Decrease LPO; Increase sperm motility and viability | NA | [54] |
| Achillea millefolium, 3 mg/L | Undefined extender, 8% glycerol | Increase sperm motility and viability | NA | [61] |
| N-acetyl-L-cysteine, 5 mmol/L and SOD, 200 U/mL | EK extender, 6% DMA | Decrease LPO; Increase sperm motility, viability, and mitochondria membrane potential | NA | [62] |