Fig. 3.

A) Top 10 sequence variants that significantly increase over time in Berlin. The mutations were pooled over locations of four different wastewater treatment plants and daytime and sorted by decreasing coefficients from linear models. Statistical significance was evaluated by a t-test using p ≤ 0.05 as cutoff. Only samples passing the sample quality scoring (>90 % reference genome coverage) were used. There was no sampling between June 11 and September 19, 2021. B) Top 10 sequence variants that significantly increase over time in New York City (NYC) (2021). The mutations were pooled over locations of 14 different wastewater treatment plants in NYC and daytime and sorted by decreasing coefficients from linear models. Statistical significance was evaluated by a t-test using p ≤ 0.05 as cutoff.