Figure 2.

Long-term passage of organoids in vitro and maintain tumour heterogeneity. (A) Bright field shows the morphology of organoids with different passage times and the proliferation ability of organoids. Scale bar, 250 mm. (B) The proliferation curve of organoids tested by CellTiter-Glo 3D cell viability assay proves that the cultured organoids can survive and inherit for a long time. (C) The morphology of individual BCOs with different subtype. Scale bar, 100 mm. (D) Morphological characteristics of tumour tissue and organoids of patients who achieved pCR. The HE stain of puncture samples and surgery tissues of patients who reached pCR after neoadjuvant chemotherapy, Scale bar, 100 mm.