Table 2.
The main components of human PDO culture medium.
| Regent name | Company | Cat No. | Stock solution | Solvent | Final concentration |
|---|---|---|---|---|---|
| Advanced DMEM/F12 | Gbico | 10565018 | – | – | 1× |
| Penicillin/streptomycin | thermo | 15140122 | 100× | – | 100 U·ml−1 / 100 mg·ml−1 |
| Hepes | Sigma | H3784-500G | – | ddH2O | 10 mM |
| GlutaMax 100× | Invitrogen | 12634034(35050061) | 100× | – | 1× |
| B27 supplement | Gibco | 17504044 | 50× | – | 1× |
| R-Spondin 1 | Peprotech | 120-38-100 | 250 ng·ul−1 | PBS | 250 ng·ml−1 |
| Neuregulin 1, recombinant human Heregulin beta 1 | Peprotech | 100-03-50 | 5 μM | PBS | 5 nM |
| FGF 7, recombinant human KGF | Peprotech | 100-19-50 | 5 ng·ul−1 | PBS | 5 ng·ml−1 |
| FGF 10 | Peprotech | 100-26-50 | 20 ng·ul−1 | PBS | 20 ng·ml−1 |
| Noggin | Peprotech | 120-10C-50 | 100 ng·ul−1 | PBS | 100 ng·ml−1 |
| N-Acetylcysteine | Sigma | A9165-5g | 1.25 M | PBS | 1.25 mM |
| Nicotinamide | Sigma | N0636 | 5 M | PBS | 5 mM |
| Primocin | Invivogen | ant-pm-1 | 50 ng·ul−1 | PBS | 50 mg·ml−1 |
| Y-27632 | stemcell | 72304 | 5 M | PBS | 5 mM |
| A83-01 | Stemcell | 72022 | 5 M | DMSO | 500 nM |
| SB202190 | Sigma | S7067 | 5 M | DMSO | 500 nM |
| EGF, human epidermal growth factor | Peprotech | AF-100-15-100 | 5 ng·ul−1 | PBS | 5 ng·ml−1 |
Note: The main components of human PDO culture medium and their concentration, including some important cytokines.