FIG. 5.

Posttranscriptional RsmA-dependent regulation of P. aeruginosa translational hcn′-′lacZ fusions. Each reporter was constructed in the vector pME6010 as described in Materials and Methods. The hcn region is indicated in boldface; the 3-bp insertion in the hcn RBS of pME3860 is boxed; artificially introduced restriction sites are marked with *. β-Galactosidase activities were determined in P. aeruginosa PAO1 wild-type (wt), PAZH13 (rsmA mutant), and PAO1(pME3849) (rsmA⁺⁺), grown in NYB to an OD₆₀₀ of 1.0 to 1.2. Activities are the means ± standard deviation from three measurements. n.d., not determined.