TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or phenotype | Reference or origin |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Wild type | Holloway Collection |
| PAZH13 | rsmA deletion mutant, derivative of PAO1 | This study |
| Plasmids | ||
| pBluescript II KS | Cloning vector; ColE1 replicon; Apr | Stratagene |
| pLPL105 | pBluescript II KS containing lasRI genes on a 2.4-kb BamHI/XhoI fragment | 28 |
| pME6001 | Cloning vector derived from pBRR1MCS, Gmr | 8 |
| pME6010 | pACYC177-pVS1 shuttle vector, Tcr | 8 |
| pME3826 | pME6010 with a 0.78-kb hcnA upstream fragment and a translational hcnA′::′lacZ fusion containing the first nine hcnA codons (EcoRV site) | 22 |
| pME3843 | pME6010 containing a 1.09-kb BglII/PstI ptac-hcn fragment and a translational hcnA′::′lacZ fusion containing the first 9 hcnA codons (EcoRV site) | 2 |
| pME3846 | pME6010 with a 666-bp rhlI upstream fragment and a translational rhlI′::′lacZ fusion containing the first 14 rhlI codons | This study |
| pME3849 | pME6001 with rsmA gene on a 311-bp BamHI/SmaI fragment | This study |
| pME3853 | pME6010 with 174-bp lasI upstream fragment and translational lasI′::′lacZ fusion containing first 13 lasI codons | This study |
| pME3859 | pME6010 with 194-bp rsmA upstream fragment and translational rsmA′::′lacZ fusion containing first 7 rsmA codons | This study |
| pME3860 | pME3843 derivative containing 3-bp insertion in RBS | This study |
| pMP21 | pMMB190 carrying 2-kb PstI fragment containing rhlRI gene from P. aeruginosa PAO1, Apr | 12 |
| pNM481 | ′lacZ fusion vector, Apr | 18a |
| pNM482 | ′lacZ fusion vector, Apr | 18a |