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. Author manuscript; available in PMC: 2023 Sep 29.
Published in final edited form as: Cell. 2022 Sep 29;185(20):3753–3769.e18. doi: 10.1016/j.cell.2022.09.004

Figure 5. Development of mural cells in the nascent vasculature of the second trimester human brain.

Figure 5.

A. UMAP plots indicating 15–23 GW mural cells according to subtypes, brain regions, and prenatal ages.

B. Heatmap demonstrating mitotic, classic pericyte, smooth muscle cell, and fibroblast gene expression in distinct groups.

C. Feature plots showing representative genes for the mitotic, smooth muscle cell, classic pericyte, and fibroblast subtypes of mural cells on the UMAP space.

D. RNA velocity analysis based on scRNA-seq data of 15 and 23 GW mural cells indicating the stage-dependent lineage trajectories.

E-F. RNAscope for ATP1A2 and KCNJ8 (classic pericyte markers) in 15 and 23 GW human brain sections. Arrows indicate vascular areas where the RNAscope probe was localized.

G. Immunostaining for CD248 (classic pericyte marker) in 17 GW human brain sections. Note the gradient of expression highest at the ventricular surface.

H-I. RNAscope for LUM and SERPING1 (fibroblast markers) in 15 and 23 GW human brain sections. Arrows indicate vascular areas where the RNAscope probe was localized.

J. Immunostaining for LUM in 17 GW human brain sections.

K-L. RNAscope for MYL9 and TAGLN (smooth muscle cell markers) in 15 and 23 GW human brain sections. Arrows indicate vascular areas where the RNAscope probe was localized.

M. Immunostaining for MYL9 in 17 GW human brain sections.