Table 4. Free energy values show that chemical stability is not enhanced for mutant I83 construct at [Ca2+] ≤ 50 μm.
Changes in free energy were determined by comparing the chemical stability of I83 in a calcium-free environment to the stability at a range of calcium concentrations.
| I83 WT | Ca-site Mutant | |
|---|---|---|
| ΔGchem, (kcal/mol) Ca-free | 3.35 ± 0.20 | 3.24 ± 0.14 |
| ΔGchem, (kcal/mol) pCa 6 (1 μM) | 4.02 ± 0.14 | 3.36 ± 0.13 |
| ΔGchem, (kcal/mol) pCa 4.3 (50 μM) | 4.86 ± 0.05 | 3.13 ± 0.19 |
| ΔGchem, (kcal/mol) pCa 2 (10 mM) | 5.64 ± 0.17 | 3.85 ± 0.22 |
| p-Value, 0 vs. pCa 6 (1 μM) | p < 0.01 | p > 0.20 |
| p-Value, pCa 6 vs. pCa 4.3 (50 μM) | p < 0.01 | p < 0.50 |
| p-Value, pCa 4.3 vs. pCa 2 (10 mM) | p < 0.01 | p < 0.02 |