REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Anti-DNA | Millipore | Cat #CBL186; RRID: AB_93367 |
Anti-HSP60 | Novus | Cat #NBP1-77397; RRID: AB_11004596 |
Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)] | GeneTex | Cat #GTX57154; RRID: AB_2887950 |
Anti-Flavivirus group antigen [D1-4G2-4-15 (4G2)] | Millipore | Cat #MAB10216-I; RRID: AB_827205 |
Anti-DENV NS3 protein | GeneTex | Cat #GTX124252; RRID: AB_11171668 |
Anti-human NANOG | R & D | Cat #AF1997; RRID: AB_355097 |
Anti-OCT-3/4 Antibody | Santa Cruz | Cat #sc-5279; RRID: AB_628051 |
Anti-SOX2 (D6D9) | Cell Signaling | Cat #3579; RRID: AB_2195767 |
TRA-1-60 Monoclonal Antibody | BD Biosciences | Cat #560071; RRID: AB_1645604 |
TRA-1-81 (Podocalyxin) Monoclonal Antibody | Thermo Scientific | Cat #14-8883-82; RRID: AB_891614 |
Anti-FOXJ1 | Thermo Fisher Scientific | Cat #14-9965-82; RRID: AB_1548835 |
Anti-Acetyl-α-Tubulin (Lys40) (D20G3) | Cell Signaling | Cat #5335S; RRID: AB_10544694 |
Anti-MAP2 antibody | Abcam | Cat #ab5392; RRID: AB_2138153 |
Anti-E-Cadherin (4A2) | Cell Signaling | Cat #14472S; RRID: AB_2728770 |
Anti-SARS-CoV/SARS-CoV-2 Nucleocapsid | SinoBiological | Cat #40143-R001; RRID: AB_2827974 |
Anti-NDUFA4 antibody | Abcam | Cat #ab129752; RRID: AB_11155881 |
Anti-β actin | Thermo Fisher Scientific | Cat # MA1-140; RRID: AB_2536844 |
Anti-STEM121 human antigen | Takara | Cat #Y40410; RRID: AB_2801314 |
Anti-Mucin 5AC antibody | Abcam | Cat #ab3649; RRID: AB_2146844 |
Anti- IFN-alpha/beta R1 | R & D | Cat #AF245; RRID: AB_355270 |
Anti- IFN-alpha/beta R2 | R & D | Cat #MAB4015; RRID: AB_2122619 |
Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat #A-21202; RRID: AB_141607 |
Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 | Thermo Fisher Scientific | Cat #A-21203; RRID: AB_141633 |
Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 594 conjugate | Thermo Fisher Scientific | Cat #A-21207; RRID: AB_141637 |
Donkey anti-Rabbit IgG (H + L) Secondary Antibody, Alexa Fluor 647 conjugate | Thermo Fisher Scientific | Cat #A-31573; RRID: AB_2536183 |
Donkey anti-Mouse IgG (H + L) Secondary Antibody, Alexa Fluor 647 | Thermo Fisher Scientific | Cat #A-31571; RRID: AB_162542 |
680RD Donkey anti-Mouse IgG Secondary Antibody | Li-Cor | Cat #926–68072; RRID: AB_10953628 |
800CW Donkey anti-Rabbit IgG Secondary Antibody | Li-Cor | Cat #926–32213; RRID: AB_621848 |
DAPI | Thermo Fisher Scientific | Cat# D1306; RRID:AB_2629482 |
Bacterial and virus strains | ||
Zika Virus (Strain: MR 766) | ZeptoMetrix | Cat# 0810521CF |
Zika Virus (Strain: PRVABC59) | ZeptoMetrix | Cat# 0810525CF |
SARS-CoV-2 Isolate USA-WA1/2020 | BEI Resources | Cat# NR-52281 |
Dengue virus type 2 | ATCC | Cat# VR-1584 |
Biological samples | ||
Patient samples | This paper | N/A |
Chemicals, peptides, and recombinant proteins | ||
Y-27632 | MedchemExpress | #HY-10583 |
CHIR99021 | Cayman Chemical | #13122 |
Retinoic acid | Sigma Aldrich | #R2625-500MG |
L-Ascorbic acid | Sigma Aldrich | #A4544-100G |
SB431542 | R&D Systems | #1614/50 |
Dorsomorphin dihydrochloride | R&D Systems | #3093/50 |
Dexamethasone | Sigma-Aldrich | #D4902 |
Potassium cyanide | Sigma-Aldrich | #60178 |
8-Bromo-cAMP | Sigma-Aldrich | #B7880 |
IBMX | Sigma-Aldrich | #I5879 |
DAPT | R&D Systems | #2634 |
Activin A | R&D Systems | #338-AC-500/CF |
Recombinant Human bFGF Protein | Peprotech | #100-18B-500UG |
Recombinant Human BMP-4 Protein | R & D Systems | #314-BP |
Recombinant Human FGF-10 Protein | R&D Systems | #345-FG-250 |
Critical commercial assays | ||
TruSeq Stranded mRNA LP | Illumina | #20020594 |
Seahorse XF Cell Mito Stress Test Kit | AGILENT TECHNOLOGIES | #103015-100 |
Deposited data | ||
RNA-seq | This Paper | GEO: GSE182376, GSE203401 |
Experimental models: Cell lines | ||
293T | ATCC | #CRL-11268 |
Vero E6 | ATCC | #CRL-1586 |
iPSC cell lines | Paull et al., 2015 | N/A |
Software and algorithms | ||
Rstudio | Rstudio | https://rstudio.com |
Seurat R package v3.1.4 | Butler et al., 2018 | https://satijalab.org/seurat/ |
DAVID6.8 | LHRI | https://david.ncifcrf.gov/home.jsp |
Adobe illustrator CC2017 | Adobe | https://www.adobe.com/product/photoshop.html |
Graphpad Prism 9 | Graphpad software | https://www.graphpad.com |