(A) Calculation of
14
C-paraquat retention in protoplasts at different time points. t=i: time of
14
C-paraquat protoplasts efflux (0, 10, 20, and 40 min). CPM
t=i
: mean value of
14
C-paraquat radioactivity retention in protoplasts at different time points. CPM
t=0
: mean value of
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C-paraquat radioactivity retention in protoplasts at the initial time. A
t=i
: percentage of
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C-paraquat radioactivity retention in protoplasts at different time points relative to the initial time.
(B)
Paraquat retention in the protoplasts, measured in the Col-0 and
DTX6
overexpression line (OE-13) (
Xia et al., 2021
). Paraquat retention was defined as a percentage of the
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C-paraquat loaded in the protoplasts. Values are mean ± SD (n = 3). Different letters indicate significant differences (P < 0.05; one-way ANOVA).