Fig. 4.

Representative western blots for HIF-1α (A), PI3K (B), AKT (C), and p-AKT (D) and quantitative analysis of HIF-1α (E), PI3K (F), AKT (G), p-AKT (H) protein levels of bovine muscle when stored at 4 °C for 0.5, 2, 6, 12, 24, and 36 h after treatment with 200 μM H₂O₂ (H₂O₂ group) and saline (control group). About 50 μg of protein was loaded per lane. Actin levels indicated equal protein loading per line. Protein expression levels are expressed as the proportion of abundance of the samples and actin. Data were expressed as mean ± standard error (n = 6). The capital letters and lowercase letters represent the significant difference (P < 0.01) in the same group at different times, respectively. * (P < 0.05) represents the difference between the two groups at the same time point. HIF-1α: hypoxia-inducible factor-1α, PI3K: phosphatidylinositol 3-kinase, AKT: serine-threonine kinase, and p-AKT: phosphorylated AKT.