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不同规格培养皿所需的转染试剂体积
Volume of transfection reagents in the different culture plates
| Plates (well/plate) | Area (cm2/well) | Tube1 (μL/well) | Tube2 (μL/well) | (μL/well) | |||||
| 5 μmol/L siRNA | Opti-MEN | DharmaTECT | Opti-MEN | Complete media | Total volume | ||||
| 12 | 4 | 5 | 95 | 0.5 | 99.5 | 800 | 1000 | ||
| 6 | 10 | 10 | 190 | 1 | 199 | 1600 | 2000 | ||
| 1 | 60 | 40 | 760 | 4 | 796 | 6400 | 8000 | ||
