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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: J Leukoc Biol. 2021 Nov 26;112(2):257–271. doi: 10.1002/JLB.3A0218-069RR

Fig. 3: Lack of contribution of Type I interferons to IRF3-mediated suppression of the IFNγ-activated state in primed BMMs.

Fig. 3:

(A) Ifnar−/− BMMs were compared to B6 BMMs using the L. pneumophila growth/restriction assay as described in Fig. 1. Data represent three biological replicates (mice) per genotype, and error bars represent the standard error of the mean across five technical replicates (wells). p-values indicate the significance of the effect of genotype on bacterial growth over time under the conditions in each sub-panel, based on a linear mixed-effect model.

(B) Irf3−/−/Bcl2L12−/−/Irf7−/− (labeled IRF3/IRF7 DKO) and B6 BMMs were used in the L. pneumophila growth/restriction assay as described in Fig. 1, and were additionally treated with 50U IFNβ or mock-treated (vehicle) starting at 2hpi. Error bars represent the standard error of the mean across four to five technical replicates (wells). p-values indicate the significance of the effect of IFNβ treatment on bacterial growth over time under the conditions in each sub-panel, based on a linear mixed-effect model.