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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: J Leukoc Biol. 2021 Nov 26;112(2):257–271. doi: 10.1002/JLB.3A0218-069RR

Fig. 4: Stat1 phosphorylation is not enhanced in IRF3-deficient BMMs after IFNγ priming.

Fig. 4:

Irf3−/−/Bcl2L12−/− (labeled KO) and B6 BMMs were unprimed or were primed with 10U/ml IFNγ, and infected with L. pneumophila. Cytoplasmic protein extracts were collected at the indicated timepoints after infection, and analyzed by Western blotting for STAT1pY701, STAT1pS727, total STAT1, and β-actin. Results are representative of three independent experiments. The slight increase in STAT1pY701 seen in IFNγ-primed Irf3−/−/Bcl2L12−/− BMMs relative to B6 BMMs at 0hpi was not seen in the other two experiments, and is considered to be insignificant.