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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: J Leukoc Biol. 2021 Nov 26;112(2):257–271. doi: 10.1002/JLB.3A0218-069RR

Fig. 5: Effect of L. pneumophila infection on IRF3 phosphorylation and distribution in IFNγ-primed vs. unprimed BMMs.

Fig. 5:

(A) B6 BMMs were primed with 10U/ml IFNγ and infected with L. pneumophila. Nuclear protein extracts were collected at the indicated timepoints after infection, and analyzed by Western blotting for murine IRF3pSer388 and TBP. Data are representative of three independent experiments.

(B) Immunofluorescence microscopy analysis of IRF3 localization in B6 BMMs primed with 0, 10, or 100 U/ml IFNγ, then fixed 2 hours after infection with L. pneumophila or stimulation with LPS.

(C) B6 BMMs were primed with 10U/ml IFNγ and infected with L. pneumophila. Cytoplasmic protein extracts were collected at the indicated timepoints after infection, and analyzed by Western blotting for IKKεpSer172, TBK1pSer172, TBK1, and β-actin.

(D) B6 BMMs were stimulated with 10U/ml IFNγ and infected with L. pneumophila. Cytoplasmic protein extracts were collected at the indicated timepoints after infection, and analyzed by Western blotting for murine IRF3pSer388, IRF3, and β-actin.