Figure 5.

Delivery of macrophages from aged ambulatory donors to aged recipients following hindlimb unloading. Ex vivo force production of soleus muscles from aged mice during early recovery (4 days) following hindlimb unloading that received intramuscular injections of macrophages from ambulatory aged mice (Mac) or PBS (Vehicle) into contralateral hindlimbs. A–D: muscles were subjected to a single-pulse stimulation to induce a twitch contraction for the measurement of specific force (A), rate of contraction (B), or rate of relaxation (C). D: raw force tracings from a single-pulse stimulation that induced a twitch contraction. E: specific force was measured across a range of stimulation frequencies from 10 to 200 Hz. F: raw force tracings from a 100 Hz stimulation. G: soleus mass of aged mice after receiving hindlimb injection of PBS (Vehicle) or macrophages from ambulatory aged mice (Mac). A–C: individual data points are shown and connected with respective contralateral limbs and the mean is represented as a bar. E: data points are shown as means ± SE. Paired t tests (A–C) or a two-way ANOVA with Sidaks multiple-comparisons test (E) was used. n = 8/group.