Figure 1.

Loss of hepatic SEL1L predisposes mice to chemically induced tumors

(A) Body weight curve for male mice over 22 months (n = 8 per group).

(B–J) Male mice were injected i.p. with 25 mg/kg diethylnitrosamine (DEN) at 2 weeks of age, placed on high-fat diet (HFD) at 4 weeks of age, and sacrificed at 6–7 months of age: (B) Schematic outline; (C and D) Representative images of liver (C, arrows: visible tumors), and external visible tumor count (D) (n = 5–7 per group); (E and F) Western blot analysis of tumor and normal liver tissue with quantitation shown below the blot after normalization to the loading control HSP90 (n = 2 per group, 2 independent repeats); (G–J) Representative images of H&E of normal liver, adenoma, and carcinoma (G and H, arrows: expanded hepatic parenchyma (1) with compressed adjacent tissue (2), asterisks: dense cellular packing), cyclin D1 (I), and KI67 (J) staining at the end of experiment, with quantitation shown in Figures S1A and S1B (n = 5–7 per group). Values, mean ± SEM; ∗∗, p < 0.01; ∗∗∗, p < 0.001 by Student’s t test.