REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
KI67	Abcam	ab16667
CYCLIND1	Abcam	ab16663
c-MYC	MilliporeSigma	C3956
V5	Invitrogen	R960-25
HA	MilliporeSigma	H9658
WNT5A	Proteintech	55184-1-AP
WNT5B	Abclonal	A8313
WNT1	Proteintech	27935-1-AP
E-CADHERIN	BD	610181
α-TUBULIN	Santa Cruz	sc-5286
HSP90	Abcam	ab13492
CALNEXIN	Enzo	ADI-SPA-860-F
SEL1L	Abcam	ab78298
HRD1 (for Western blot)	Dr. Richard Wojcikiewicz, SUNY Upstate Medical University	Pearce et al., 2007
HRD1 (for IHC)	MilliporeSigma	HPA024300
Bacterial and virus strains
AAV8	University of Michigan Vector Core	pAAV8-D(+)-U6-siRNA-CMV-GFP
Biological samples
Frozen biopsies of hepatocellular carcinoma tumor samples and adjacent normal liver tissue	University of Michigan Pathology	https://www.pathology.med.umich.edu/
Human hepatocellular carcinoma tissue section microarray	US Biomax, Inc.	HLivH180Su14
Chemicals, peptides, and recombinant proteins
DEN	MilliporeSigma	N0258
CCL4	MilliporeSigma	289116
Recombinant Wnt5A	R&D systems	645-WN-010
Critical commercial assays
TMTsixplex™ Isobaric Label Reagent Set	ThermoFisher	PI90061
Deposited data
Proteomics analysis of purified microsomes	PRIDE ProteomeXchange	PXD035243
cDNA profiling data of total liver	GEO	GSE118658
Liver Hepatocellular Carcinoma TCGA Pan Cancer Atlas dataset	cBio Portal for Cancer Genomics	https://www.cbioportal.org/
Experimental models: Cell lines
HEK293T	ATCC	CRL-3216
HepG2	ATCC	HB-8065
Huh7	Dr. Kezhong Zhang, Wayne State University	Dr. Christopher M. Schonhoff, Tufts University Cummings School of Veterinary Medicine
Experimental models: Organisms/strains
Mice: Sel1Lf/f;Alb-Cre/+ and Sel1Lf/f	Dr. Ling Qi, University of Michigan	Bhattacharya et al. (2018)
Mice: Hrd1f/f;Alb-Cre/+ and Hrd1f/f	Dr. Deyu Fang, Northwestern University	Wei et al. (2018)
Oligonucleotides
Primers and siRNA	See Table S2 for sequences	N/A
Recombinant DNA
Active-WNT5A-V5	Addgene	43813
pAAV8-D(+)-U6-siRNA-CMV-GFP	Addgene	85451
HRD1 plasmid (MYC-tagged WT and mutant C2A)	Dr. Yihong Ye, NIDDK	Kikkert et al. (2004)
pcDNA3-HA-Ub	Dr. Hideki Nishitoh, University of Miyazaki	Sun et al. (2015)
Software and algorithms
Kaplan-Meier Plotter	http://kmplot.com/analysis,	Menyhart et al. (2018)
Gene Expression Profiling Interactive Analysis (GEPIA)	http://gepia.cancer-pku.cn/index.html	Tang et al. (2017)