Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 10;13:5986. doi: 10.1038/s41467-022-33483-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Slice through a representative cryo-electron tomogram of a lamella milled through a PV-infected cell at 6 h p.i., revealing PV-induced SM (single membranes) and ALM (autophagy-like membranes) proliferation. Yellow arrowheads indicate densities tethering intracellular empty capsids and darker RNA-loaded virions to membranes. Red arrowhead indicates a virion enclosed inside a DMV (double-membrane vesicle), proximal but not tethered to the membrane. b Segmentation of the tomogram presented in (a). Color labels are defined for each structure. Empty capsids (pink) and RNA-loaded virions (red) are represented by their subtomogram averages. c, d Magnified view of an empty capsid and RNA-loaded virion tethered to a DMV shown in (a) with black boxes. e Percentage of empty (n = 223) and RNA-loaded virions (n = 281) observed on SM and ALM. f Subtomogram average of the tethered viral capsid at 70 Å resolution. Its height and width are marked, and its molecular mass is estimated to be ~230 kDa. g Percentage of (n) virions observed on the outside (n = 520, blue) and inside (n = 22, red) of ALM having a visible tether to a membrane as indicated in the inset. h Percentage of empty capsids observed in (n) tomograms of untreated (n = 43) and Hydantoin-treated cells (n = 14) at 6 h p.i., as measured by template matching. Horizontal lines represent the average. i Cryo-electron tomogram of a PV-infected, Hydantoin-treated cell at 6 h p.i., containing several empty capsids which are not tethered to the surrounding membranes. j Segmentation of the tomogram in (i). Color labels for each structure are the same in (b) and empty capsids are represented by their subtomogram average. k Percentage of tethered virions as observed in (n) cryo-tomograms of untreated (n = 33) and Hydantoin-treated (n = 14) cells at 6 h p.i. Horizontal lines represent the average. In all graphs, each dot corresponds to one tomogram analyzed (see also Supplementary Table 2). Statistical significance by unpaired two-tailed Student’s t test: g ****p < 0.0001, h **p = 0.0138, k **p = 0.0089. Scale bars: a–i 100 nm, c, d 50 nm.